Coding
todE

Part:BBa_K2626000:Design

Designed by: Kristian Emilov   Group: iGEM18_Westminster_UK   (2018-10-09)


todE coding sequence encoding 3-methylcatechol 2,3-dioxygenase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 344
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 394
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 419


Design Notes

Coding sequence was scanned for illegal sites. a thymine was changed to a cytosine at position of 376. the codon was preserved to keep to the amino acid sequence identical.


Source

This specific gene, todE, originates from the toluene degradation operon, from the bacteria Pseudomonas putida F1. NCBI ref number: AB828709.1 (https://www.ncbi.nlm.nih.gov/nucleotide/AB828709.1?report=genbank&log$=nuclalign&blast_rank=3&RID=VW56XSTR013) (F1 genome: CP000712.1)

References